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Osmonauts - Dmitry Rinberg. Cracking the Olfactory Code
Abstract 1:
Title: High-speed volumetric imaging of piriform cortex during odor stimulation
Abstract: High-speed volumetric multiphoton imaging data of piriform cortex layers 2 and 3 were collected using a 16kHz resonant galvo in paralyzed but awake mice being exposed to different odor sets, in which each set parametrically varied odor distance at a particular scale (as measured in an odor space defined by more than 5,000 known odorants, using a PCA reduction of a set of more than 4,000 physiochemical features). These three sets were defined as “global” “tiled” and “clustered” depending upon inter-odor distances. Acquisition volumes spanned 210 mm in the Z axis across PCx L2 and L3. Volumes were split into 6 optical slices each spanning 35 mm of cortex. Volumes were positioned such that 2 slices resided in L2 and 4 slices resided in L3. This allowed us to monitor similarly sized populations of neurons in L2 and L3 given the approximately 3-fold lower cell density of L3 in posterior PCx. For experiments involving the global, clustered and tiled odor sets in odor-naïve animals, data was analyzed from 3 animals per odor set. In independent experiments, olfactory bulb inputs to the piriform (which reside in layer 1) were imaged in the same configuration (via homogenous viral delivery of GCaMP6s to bulb projection neurons); these experiments yielded >500 boutons per imaging field (x 3 mice) for the tiled odor set only.