Two solutes, with given total concentrations, Atot and Ftot, bind with an equilibrium dissociation constant of Kd. The result gives the concentrations of A, F, and AF after equilibration. Check affirms totals.
Description
Equilibration calculation used in Barta et al (2000) Barta E, Sideman S, and Bassingthwaighte JB. Facilitated diffusion and membrane permeation of fatty acid in albumin solutions. Ann Biomed Eng 28: 331-345, 2000. COMMENT: Choosing Kd = 330 gives F = AF = 270 with ATot 600 and Ftot 540 uM. Choosing Kd = 240 gives A = AF = 270 with ATot 600 and Ftot 540 uM.
Equations
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Barta E, Sideman S, and Bassingthwaighte JB. Facilitated diffusion and membrane permeation of fatty acid in albumin solutions. Ann Biomed Eng 28: 331-345, 2000.
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